Rabbit Anti-Human PDS-1/Securin Polyclonal Antibody | MyBioSource MBS301928 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human PDS-1/Securin Polyclonal Antibody

catalog :

MBS301928

quantity :

0.1 ml

price :

170 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, cow

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301928

products type :

Antibody

products full name :

Rabbit Anti-Human PDS-1/Securin Polyclonal Antibody

products short name :

PDS-1/Securin

other names :

insulin-like growth factor II isoform 1 preproprotein; Insulin-like growth factor II; insulin-like growth factor II; T3M-11-derived growth factor; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor type 2; insulin-like growth factor 2; Somatomedin-A; T3M-11-derived growth factorCleaved into the following 3 chains:Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin

products gene name :

PDS-1

other gene names :

IGF2; IGF2; IGF-II; PP9974; C11orf43; IGF-II

uniprot entry name :

IGF2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine

sequence length :

180

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Rectal Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from internal region of human PDS-1 protein. Control Tissue: Rectal Carcinoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 9232. Pathway: Hormones

products description :

Securin is an anaphase inhibitor and plays an important role in DNA damage and spindle check point pathways. It inhibits sister chromatid separation by binding and inhibiting Esp 1, a cystein protease that causes cleavage of the cohesin Scc 1 that binds the sister chromatids together. It is responsible for targeting of Esp 1 to the nucleus and its binding to the spindle. Degradation of securin occurs shortly before anaphase, which liberates Esp 1 and is a pre-requisite for anaphase entry. It is targeted for degradation by ubiquitination mediated bycyclosome / anaphase promoting complex (APC) functioning as an ubiquitin ligase. In response to DNA damage, Chk 1 phosphorylates securin to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase. Securin is highly expressed in various human tumors, including pituitary, adrenal, kidney, liver, and ovarian tumors, and in cell lines derived from pituitary tumors, breast tumors, endometrial tumors, and ovarian tumors.

ncbi gi num :

4504609

ncbi acc num :

NP_000603.1

ncbi gb acc num :

NM_000612.5

ncbi mol weight :

42 kDa

ncbi pathways :

Apoptosis Pathway 198797!!Cardiac Progenitor Differentiation Pathway 712094!!Endochondral Ossification Pathway 198812!!Hedgehog Signaling Pathway 198835!!Hemostasis Pathway 106028!!IGF1R Signaling Cascade Pathway 730346!!IRS-related Events Triggered By IGF1R Pathway 730348!!Metabolism Of Proteins Pathway 106230!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050

ncbi summary :

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

size :

0.1 ml

price :

170 USD