antibody

Rabbit Anti-Human PD-1/CD279 Polyclonal Antibody

MBS301919

0.1 ml

170 USD

polyclonal

rabbit

nonconjugated

human, mouse

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Rabbit Anti-Human PD-1/CD279 Polyclonal Antibody

PD-1/CD279

programmed cell death protein 1; Programmed cell death protein 1; programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2; programmed cell death 1; CD_antigen: CD279

PD-1/CD279

PDCD1; PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD1; Protein PD-1; hPD-1

PDCD1_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Mouse

288

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Lymph Node

Immunogen: Synthetic peptide derived from internal region of human PD-1/CD279 protein. Control Tissue: Lymph Node, Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 5133. Pathway: Apoptosis

CD279 is an immunoglobulin superfamily member, also known as Programmed Cell Death 1. Programmed Cell Death 1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. Programmed Cell Death 1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The Programmed Cell Death 1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.

167857792

NP_005009.2

NM_005018.2

32 kDa

Adaptive Immune System Pathway 366160!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Costimulation By The CD28 Family Pathway 119552!!Immune System Pathway 106386!!PD-1 Signaling Pathway 119557!!T Cell Receptor Signaling Pathway 83080!!T Cell Receptor Signaling Pathway 491

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

0.1 ml

170 USD