MyBioSource

antibody

Rabbit Anti-Human Keratin 10 Polyclonal Antibody

MBS301838

1 ml

485 USD

polyclonal

rabbit

immunohistochemistry, immunohistochemistry - paraffin section

MBS301838

Antibody

Rabbit Anti-Human Keratin 10 Polyclonal Antibody

Keratin 10

keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 10; keratin, type I cytoskeletal 10; CK-10; cytokeratin 10; keratin-10; keratin 10, type I; Cytokeratin-10; CK-10; Keratin-10; K10

KRT10; KRT10; BIE; EHK; K10; KPP; BCIE; CK10; KPP; CK-10; K10

K1C10_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Bovine, Dog

584

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Skin

Immunogen: Synthetic peptide derived from C-terminus of human keratin 10 protein. Control Tissue: Skin. Cellular Localization: Cytoplasm. Entrez Gene Code: 3858. Pathway: Signal Transduction

Cytokeratin 10 is a type I cytokeratin, expressed by suprabasal cells of stratified and cornified epithelia. Its defects are implicated in bullous congenital ichthyosiform erythroderma, epidermolytic hyperkeratosis and diffuse non-epidermolytic palomoplantar keratoderma.

195972866

NP_000412.3

NM_000421.3

56.5 kDa

Staphylococcus Aureus Infection Pathway 172846!!Staphylococcus Aureus Infection Pathway 171867

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

1 ml

485 USD