MyBioSource

antibody

Rabbit Anti-Human FOXP3 Polyclonal Antibody

MBS301811

1 ml

485 USD

polyclonal

rabbit

human, mouse, rat, dog

immunohistochemistry, immunohistochemistry - paraffin section

MBS301811

Antibody

Rabbit Anti-Human FOXP3 Polyclonal Antibody

FOXP3

forkhead box protein P3 isoform b; Forkhead box protein P3; forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; scurfin; forkhead box P3; Scurfin

FOXP3

FOXP3; FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; IPEX

FOXP3_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat

396

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Tonsil

Immunogen: Synthetic peptide derived from C-terminus of human FOXP3 protein. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 50943. Pathway: Transcriptional Regulators

FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

167466190

NP_001107849.1

NM_001114377.1

50 kDa

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!IL2 Signaling Events Mediated By STAT5 Pathway 137988!!Inflammatory Bowel Disease (IBD) Pathway 842771!!Inflammatory Bowel Disease (IBD) Pathway 842797

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

1 ml

485 USD