Rabbit Anti-Mouse F4/80 Polyclonal Antibody | MyBioSource MBS301807 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Mouse F4/80 Polyclonal Antibody

catalog :

MBS301807

quantity :

0.1 mL (Concentrate)

price :

180 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301807

products type :

Antibody

products full name :

Rabbit Anti-Mouse F4/80 Polyclonal Antibody

products short name :

F4/80

other names :

EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor

products gene name :

F4/80

other gene names :

Emr1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7; Gpf480

uniprot entry name :

EMR1_MOUSE

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Mouse, Rat

sequence length :

931

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols.Antigen Retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Human Lung, Mouse Liver

other info2 :

Immunogen: Synthetic peptide derived from N-terminus of mouse F4/80 protein. Control Tissue: Lung. Cellular Localization: Membrane. Entrez Gene Code: 13733. Pathway: Macrophage

products description :

F4/80 antigen is a 160kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins which shares 68% overall amino acid identity with human EGF module containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells

ncbi gi num :

33859546

ncbi acc num :

NP_034260.1

ncbi gb acc num :

NM_010130.4

ncbi mol weight :

160 kDa

ncbi pathways :

GPCRs, Class B Secretin-like Pathway (198364); Non-odorant GPCRs Pathway (198395)

uniprot summary :

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; GPCR, family 2; Membrane protein, integral; Receptor, GPCR. Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding. Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction

size1 :

0.1 mL (Concentrate)

price1 :

180 USD

size2 :

7 mL (RTU)

price2 :

290

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

495