Rabbit Anti-Human c-erbB-2 (Paired 1248) Polyclonal Antibody | MyBioSource MBS301755 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human c-erbB-2 (Paired 1248) Polyclonal Antibody

catalog :

MBS301755

quantity :

7 ml

price :

285 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301755

products type :

Antibody

products full name :

Rabbit Anti-Human c-erbB-2 (Paired 1248) Polyclonal Antibody

products short name :

c-erbB-2

other names :

receptor tyrosine-protein kinase erbB-2 isoform b; Receptor tyrosine-protein kinase erbB-2; receptor tyrosine-protein kinase erbB-2; c-erb B2/neu protein; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; proto-oncogene Neu; proto-oncogene c-ErbB-2; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; erb-b2 receptor tyrosine kinase 2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340

other gene names :

ERBB2; ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; HER2; MLN19; NEU; NGL; MLN 19

uniprot entry name :

ERBB2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Chicken, Dog, Frog, Hamster, Mouse, Rat

sequence length :

1225

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from the region of human c-erbB-2 that contains non-phosphorylated tyrosine 1248. Control Tissue: Breast Carcinoma. Cellular Localization: Membrane. Entrez Gene Code: 2064. Pathway: EGFR Family

products description :

c-erbB-2 is a 185kDa transmembrane tyrosine kinase which belongs to the epidermal growth factor receptor (EGFR) family. This family comprises four homologous receptors ERFR, c-erbB-2, c-erbB-3 and c-erbB-4. The HER2 gene is located on chromosome 17 at q21. Activation of c-erbB2 through autophosphorylation at tyrosine residues, is initiated by either homo- or heterodimerization and triggers intracellular signaling events, which are crucial for cell growth, differentiation and survival. This antibody labels activated c-cerbB-2 that is non-phosphorylated at tyrosine residue.

ncbi gi num :

54792098

ncbi acc num :

NP_001005862.1

ncbi gb acc num :

NM_001005862.2

ncbi mol weight :

185 kDa

ncbi pathways :

Adaptive Immune System Pathway 366160!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Axon Guidance Pathway 105688!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Central Carbon Metabolism In Cancer Pathway 1059538

ncbi summary :

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

size :

7 ml

price :

285 USD