catalog number :
MBS301734
products full name :
Rabbit Anti-Human CD19 Polyclonal Antibody
products short name :
CD19
other names :
B-lymphocyte antigen CD19 isoform 1; B-lymphocyte antigen CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12; differentiation antigen CD19; CD19 molecule; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19
products gene name :
CD19
other gene names :
CD19; CD19; B4; CVID3
uniprot entry name :
CD19_HUMAN
reactivity :
Human. Predicted Reactivity: Bovine, Pig
form :
1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Tonsil
other info2 :
Immunogen: Synthetic peptide derived from C-terminus of human CD19 protein. Control Tissue: Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 930. Pathway: Stem Cell
products description :
CD19 is expressed in B lymphocytes and B-cell lymphoma. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. CD19 is negative in T lymphocytes, plasma cells, and their tumors
ncbi acc num :
NP_001171569.1
ncbi gb acc num :
NM_001178098.1
ncbi pathways :
Adaptive Immune System Pathway 366160!!Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway 576249!!B Cell Receptor Signaling Pathway 198909!!B Cell Receptor Signaling Pathway 83081!!B Cell Receptor Signaling Pathway 492!!BCR Signaling Pathway 138058!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Disease Pathway 530764