Rabbit Anti-Human 14-3-3 zeta Polyclonal Antibody | MyBioSource MBS301705 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human 14-3-3 zeta Polyclonal Antibody

catalog :

MBS301705

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, chicken, mouse, rat

application :

immunohistochemistry - paraffin section, western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301705

products type :

Antibody

products full name :

Rabbit Anti-Human 14-3-3 zeta Polyclonal Antibody

products short name :

14-3-3 zeta

other names :

14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; 14-3-3 delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; epididymis luminal protein 4; epididymis secretory protein Li 3; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; Protein kinase C inhibitor protein 1; KCIP-1

products gene name :

14-3-3 zeta

other gene names :

YWHAZ; YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta; KCIP-1

uniprot entry name :

1433Z_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Mouse, Rat

sequence length :

245

form :

0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1%BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blotting (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Tonsil, Breast Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: 293T Cell Lysate

other info2 :

Immunogen: Synthetic peptide derived from internal region of human 14-3-3 zeta. Control Tissue: Breast Carcinoma, Tonsil. Cellular Localization: Cytoplasm. Entrez Gene Code: 7534. Pathway: Signal Transduction

products description :

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, theta, epsilon, zeta, sigma, and nu. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation and activation/repression of enzyme activity.

ncbi gi num :

208973238

ncbi acc num :

NP_001129171.1

ncbi gb acc num :

NM_001135699.1

ncbi mol weight :

28 kDa

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Adaptive Immune System Pathway 366160!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463!!Class I PI3K Signaling Events Mediated By Akt Pathway 138020

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

size :

7 ml

price :

220 USD

more info or order :

MyBioSource product webpage