Rabbit Anti-Human ZAP-70 Polyclonal Antibody | MyBioSource MBS301698 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human ZAP-70 Polyclonal Antibody

catalog :

MBS301698

quantity :

1 ml

price :

485 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

immunohistochemistry - paraffin section, western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301698

products type :

Antibody

products full name :

Rabbit Anti-Human ZAP-70 Polyclonal Antibody

products short name :

ZAP-70

other names :

tyrosine-protein kinase ZAP-70 isoform 1; Tyrosine-protein kinase ZAP-70; tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; syk-related tyrosine kinase; zeta-chain associated protein kinase, 70kD; zeta-chain (TCR) associated protein kinase 70kDa; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

products gene name :

ZAP-70

other gene names :

ZAP70; ZAP70; SRK; STD; TZK; STCD; ZAP-70; SRK

uniprot entry name :

ZAP70_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

619

form :

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Jurkat Cell Lysate

other info2 :

Immunogen: Synthetic peptide corresponding to C-terminus of human ZAP-70. Control Tissue: Tonsil. Cellular Localization: Cytoplasm. Entrez Gene Code: 7535. Pathway: Cancer Markers

products description :

Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression is also seen in a subset of Chronic Lymphocytic Leukemia (CLL).

ncbi gi num :

31455611

ncbi acc num :

NP_001070.2

ncbi gb acc num :

NM_001079.3

ncbi mol weight :

70 kDa

ncbi pathways :

Adaptive Immune System Pathway 366160!!B Cell Receptor Signaling Pathway 198909!!Generation Of Second Messenger Molecules Pathway 106417!!Immune System Pathway 106386!!Inflammatory Response Pathway 198766!!Interferon Type I Pathway 920993!!NF-kappa B Signaling Pathway 634527!!Natural Killer Cell Mediated Cytotoxicity Pathway 83079!!Natural Killer Cell Mediated Cytotoxicity Pathway 490!!Primary Immunodeficiency Pathway 83125

ncbi summary :

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

size :

1 ml

price :

485 USD

more info or order :

MyBioSource product webpage