Rabbit Anti-Human Wnt-1 Polyclonal Antibody | MyBioSource MBS301691 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Wnt-1 Polyclonal Antibody

catalog :

MBS301691

quantity :

0.1 ml

price :

165 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, dog, mouse

application :

immunohistochemistry, western blot, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301691

products type :

Antibody

products full name :

Rabbit Anti-Human Wnt-1 Polyclonal Antibody

products short name :

WNT-1

other names :

proto-oncogene Wnt-1; Proto-oncogene Wnt-1; proto-oncogene Wnt-1; proto-oncogene Int-1 homolog; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Proto-oncogene Int-1 homolog

products gene name :

WNT-1

other gene names :

WNT1; WNT1; INT1; OI15; BMND16; INT1

uniprot entry name :

WNT1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Dog, Frog, Hamster, Mouse, Pig

sequence length :

370

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from N-terminus of human WNT-1. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 7471. Pathway: Signal Transduction

products description :

Wnt-1 is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neurol tube and adult spermatids.

ncbi gi num :

4885655

ncbi acc num :

NP_005421.1

ncbi gb acc num :

NM_005430.3

ncbi mol weight :

42 kDa

ncbi pathways :

Adipogenesis Pathway 198832!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!C-MYB Transcription Factor Network Pathway 138073!!Class B/2 (Secretin Family Receptors) Pathway 106378!!DNA Damage Response (only ATM Dependent) Pathway 198827!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Developmental Biology Pathway 477129!!Disease Pathway 530764!!GPCR Ligand Binding Pathway 161020

ncbi summary :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

size :

0.1 ml

price :

165 USD

more info or order :

MyBioSource product webpage