Rabbit Anti-Human Ubiquitin Polyclonal Antibody | MyBioSource MBS301671 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Ubiquitin Polyclonal Antibody

catalog :

MBS301671

quantity :

0.1 ml

price :

155 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, chicken

application :

immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301671

products type :

Antibody

products full name :

Rabbit Anti-Human Ubiquitin Polyclonal Antibody

products short name :

Ubiquitin

other names :

polyubiquitin-B; Polyubiquitin-B; polyubiquitin-B; polyubiquitin B; ubiquitin B; N/A

other gene names :

UBB; UBB; N/A

uniprot entry name :

UBB_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat

sequence length :

229

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Pancreas, Alzheimer's Brain

other info2 :

Immunogen: Recombinant full length human ubiquitin protein. Control Tissue: Alzheimer's Brain, Pancreas. Cellular Localization: Cytoplasm, Membrane, Nucleus. Entrez Gene Code: 7314. Pathway: Proteolysis

products description :

Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

ncbi gi num :

11024714

ncbi acc num :

NP_061828.1

ncbi gb acc num :

NM_018955.3

ncbi mol weight :

8.5 kDa

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway 1127621!!APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway 1127616!!APC Truncation Mutants Have Impaired AXIN Binding Pathway 1127617!!APC-Cdc20 Mediated Degradation Of Nek2A Pathway 1127551!!APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway 105832!!APC/C:Cdc20 Mediated Degradation Of Securin Pathway 105833!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 105835!!APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway 1127549

ncbi summary :

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

size :

0.1 ml

price :

155 USD

more info or order :

MyBioSource product webpage