Mouse Anti-Human TTF-1 (Thyroid Transcription Factor-1) Monoclonal Antibody (Clone 8G7G3/1) | MyBioSource MBS301659 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human TTF-1 (Thyroid Transcription Factor-1) Monoclonal Antibody (Clone 8G7G3/1)

catalog :

MBS301659

quantity :

0.1 mL (Concentrate)

price :

195 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8G7G3/1

reactivity :

human, mouse, rat, chicken

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301659

products type :

Antibody

products full name :

Mouse Anti-Human TTF-1 (Thyroid Transcription Factor-1) Monoclonal Antibody (Clone 8G7G3/1)

products short name :

TTF-1

other names :

homeobox protein Nkx-2.1 isoform 1; Homeobox protein Nkx-2.1; homeobox protein Nkx-2.1; NK-2 homolog A; homeobox protein NK-2 homolog A; thyroid nuclear factor 1; thyroid transcription factor 1; thyroid-specific enhancer-binding protein; NK2 homeobox 1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1; Thyroid-specific enhancer-binding protein; T/EBP

products gene name :

TTF-1

other gene names :

NKX2-1; NKX2-1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; T/EBP; TITF1; TTF-1; NKX2.1; NKX2A; TITF1; TTF1; TTF-1; T/EBP

uniprot entry name :

NKX21_HUMAN

clonality :

Monoclonal

isotype :

IgG1/k

clone :

8G7G3/1

host :

Mouse

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Dog, Frog, Mouse, Rat

sequence length :

401

form :

0.1 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Normal Thyroid, Lung

other info2 :

Immunogen: Rat TTF-1 recombinant protein. Control Tissue: Lung, Thyroid. Cellular Localization: Nucleus. Entrez Gene Code: 7080. Pathway: Cancer Markers

products description :

Thyroid transcription factor-1 (TTF-1) is a member of the NKx2 family of homeodomain transcription factors. It is expressed in the epithelial cells of the thyroid gland and lung and their tumors.

ncbi gi num :

118766339

ncbi acc num :

NP_001073136.1

ncbi gb acc num :

NM_001079668.2

ncbi mol weight :

41,737 Da

ncbi pathways :

FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

ncbi summary :

This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]

uniprot summary :

NKX2-1: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC); also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). This syndrome include neurological, thyroid, and respiratory problems. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 14q13. Cellular Component: nucleoplasm; transcription factor complex; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; axon guidance; positive regulation of transcription, DNA-dependent; neuron migration; response to hormone stimulus; rhythmic process; globus pallidus development; forebrain dorsal/ventral pattern formation; locomotory behavior; positive regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; Leydig cell differentiation; phospholipid metabolic process; forebrain development; thyroid gland development; anatomical structure formation; oligodendrocyte differentiation; forebrain neuron fate commitment; negative regulation of cell migration; hippocampus development; induction; cerebral cortex GABAergic interneuron differentiation; cerebral cortex cell migration; pituitary gland development; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; lung development; endoderm development. Disease: Thyroid Carcinoma, Papillary; Chorea, Benign Hereditary; Choreoathetosis And Congenital Hypothyroidism With Or Without Pulmonary Dysfunction

size1 :

0.1 mL (Concentrate)

price1 :

195 USD

size2 :

7 mL (RTU)

price2 :

280

size3 :

0.5 mL (Concentrate)

price3 :

385

size4 :

1 mL (Concentrate)

price4 :

565