product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Thymidylate Synthase (TS) Polyclonal Antibody
catalog :
MBS301656
quantity :
7 ml
price :
285 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, rat, mouse
application :
immunohistochemistry - paraffin section, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS301656
products type :
Antibody
products full name :
Rabbit Anti-Human Thymidylate Synthase (TS) Polyclonal Antibody
products short name :
Thymidylate Synthase
other names :
thymidylate synthase; Thymidylate synthase; thymidylate synthase; TSase; thymidylate synthetase; N/A
products gene name :
TS
other gene names :
TYMS; TYMS; TS; TMS; HST422; TS; TS; TSase
uniprot entry name :
TYSY_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Bovine, Mouse, Rabbit, Rat
sequence length :
313
form :
7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Colon Carcinoma
other info2 :
Immunogen: Synthetic peptide corresponding to human TS. Control Tissue: Colon Carcinoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 7298. Pathway: DNA Replication and Repair
products description :
Thymidylate Synthase (TS) (EC 2.1.1.45) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been demonstrated in human colorectal, gastric, head and neck, and breast carcinomas.
ncbi gi num :
4507751
ncbi acc num :
NP_001062.1
ncbi gb acc num :
NM_001071.2
ncbi mol weight :
36 kDa
ncbi pathways :
Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!E2F Mediated Regulation Of DNA Replication Pathway 105778!!E2F Transcription Factor Network Pathway 137934!!Fluoropyrimidine Activity Pathway 198778!!G1/S Transition Pathway 105769!!G1/S-Specific Transcription Pathway 105776!!Integrated Pancreatic Cancer Pathway 711360!!Metabolic Pathways 132956!!Metabolism Pathway 477135
ncbi summary :
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]
size :
7 ml
price :
285 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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