Rabbit Anti-Human Thyroid Hormone Receptor alpha1/alpha2 Polyclonal Antibody | MyBioSource MBS301635 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Thyroid Hormone Receptor alpha1/alpha2 Polyclonal Antibody

catalog :

MBS301635

quantity :

0.1 mL (Concentrate)

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301635

products type :

Antibody

products full name :

Rabbit Anti-Human Thyroid Hormone Receptor alpha1/alpha2 Polyclonal Antibody

products short name :

Thyroid Hormone Receptor alpha1/alpha2

other names :

thyroid hormone receptor alpha isoform 3; Thyroid hormone receptor alpha; thyroid hormone receptor alpha; EAR-7; ERBA-related 7; V-erbA-related protein 7; c-erbA-alpha; nuclear receptor subfamily 1 group A member 1; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid normone nuclear receptor alpha variant 1; triiodothyronine receptor; thyroid hormone receptor, alpha; Nuclear receptor subfamily 1 group A member 1; V-erbA-related protein 7; EAR-7; c-erbA-1; c-erbA-alpha

other gene names :

THRA; THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1; EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7

uniprot entry name :

THA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat

sequence length :

451

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:150. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:150. IHC Positive Control: Thyroid

other info2 :

Immunogen: Synthetic peptide derived from N-terminus of human TR-alpha 1 protein. Control Tissue: Thyroid. Cellular Localization: Nucleus. Entrez Gene Code: 7067. Pathway: Hormones

products description :

Thyroid hormone receptors (TRs) are ligand-dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T-3).

ncbi gi num :

300116307

ncbi acc num :

NP_001177847.1

ncbi gb acc num :

NM_001190918.1

ncbi mol weight :

~50 kDa (TR- alpha1) and ~60 kDa (TR- alpha2)

ncbi pathways :

Endochondral Ossification Pathway (198812); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Thyroid Hormone Signaling Pathway (946598)

ncbi summary :

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described. Protein type: Transcription factor; Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: nucleoplasm; cytosol; nucleus. Molecular Function: protein domain specific binding; protein binding; zinc ion binding; sequence-specific DNA binding; chromatin DNA binding; TATA-binding protein binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; cytoplasmic sequestering of transcription factor; ossification; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation. Disease: Hypothyroidism, Congenital, Nongoitrous, 6

size1 :

0.1 mL (Concentrate)

price1 :

165 USD

size2 :

7 mL (RTU)

price2 :

225

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

420