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antibody

Rabbit Anti-Human SOX9 Polyclonal Antibody

MBS301594

0.1 ml

170 USD

polyclonal

rabbit

dog, mouse, human

immunohistochemistry - paraffin section, immunohistochemistry

MBS301594

Antibody

Rabbit Anti-Human SOX9 Polyclonal Antibody

SOX-9

transcription factor SOX-9; Transcription factor SOX-9; transcription factor SOX-9; SRY (sex-determining region Y)-box 9 protein; SRY-related HMG-box, gene 9; SRY (sex determining region Y)-box 9; N/A

SOX-9

SOX9; SOX9; CMD1; SRA1; CMPD1; N/A

SOX9_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Chicken, Dog, Frog, Mouse, Pig

509

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Testis

Immunogen: Recombinant protein encoding aa 1-150 of human SOX-9 protein. Control Tissue: Testis. Cellular Localization: Nucleus. Entrez Gene Code: 6662. Pathway: Stem Cell

SOX9 is a member of the family of SOX (Sry-type high mobility group box) gene that was first identified on the basis of region with high homology to that of Sry (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX9 by PKA increases its DNA binding and transcriptional activity. SOX9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibiting the expression of genes for cartilage extracellular matrix proteins. The down-regulation of Sox9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.

4557853

NP_000337.1

NM_000346.3

61 kDa

Endochondral Ossification Pathway 198812!!Neural Crest Differentiation Pathway 672460!!Spinal Cord Injury Pathway 739007!!Wnt Signaling Pathway NetPath 198799!!CAMP Signaling Pathway 1017634!!CAMP Signaling Pathway 1019520

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

0.1 ml

170 USD