product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human S100A4 Polyclonal Antibody
catalog :
MBS301562
quantity :
0.1 ml
price :
170 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat, dog
application :
immunohistochemistry - paraffin section, immunohistochemistry, western blot
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS301562
products type :
Antibody
products full name :
Rabbit Anti-Human S100A4 Polyclonal Antibody
products short name :
S100A4
other names :
protein S100-A4; Protein S100-A4; protein S100-A4; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); fibroblast-specific protein-1; leukemia multidrug resistance associated protein; malignant transformation suppression 1; placental calcium-binding protein; protein Mts1; S100 calcium binding protein A4; Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
products gene name :
S100A4
other gene names :
S100A4; S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; CAPL; MTS1
uniprot entry name :
S10A4_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Bovine, Dog, Mouse, Rat
sequence length :
101
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Tonsil, Melanoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Placenta Cell Lysate
other info2 :
Immunogen: Recombinant protein encoding full length human S100A4. Control Tissue: Melanoma, Tonsil. Cellular Localization: Cytoplasm. Entrez Gene Code: 6275. Pathway: Cancer Markers
products description :
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and inter-digitating reticulum cells in the paracortex of lymph node.
ncbi gi num :
4506765
ncbi acc num :
NP_002952.1
ncbi gb acc num :
NM_002961.2
ncbi mol weight :
12 kDa
ncbi summary :
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
size :
0.1 ml
price :
170 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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