Rabbit Anti-Human PLC beta 1 Polyclonal Antibody | MyBioSource MBS301511 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human PLC beta 1 Polyclonal Antibody

catalog :

MBS301511

quantity :

0.1 mL (Concentrate)

price :

180 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301511

products type :

Antibody

products full name :

Rabbit Anti-Human PLC beta 1 Polyclonal Antibody

products short name :

PLC beta 1

other names :

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform a; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; PLC-beta-1; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; phospholipase C-I; triphosphoinositide phosphodiesterase; phospholipase C, beta 1 (phosphoinositide-specific); PLC-154; Phosphoinositide phospholipase C-beta-1; Phospholipase C-I; PLC-I; Phospholipase C-beta-1; PLC-beta-1

products gene name :

PLC beta 1

other gene names :

PLCB1; PLCB1; PLC-I; EIEE12; PI-PLC; PLC154; PLCB1A; PLCB1B; PLC-154; KIAA0581; PLC-I; PLC-beta-1

uniprot entry name :

PLCB1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

1216

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:25. IHC Positive Control: Brain

other info2 :

Immunogen: Synthetic peptide corresponding to C-terminus of human PLC beta 1. Control Tissue: Brain. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 23236. Pathway: Synthesis and Degradation

products description :

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5- triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gemma1, PLC gemma2, PLC delta1, PLC delta2 and PLC epsilon, with molecular weights ranging from 85 to 150kDa. PLC beta1, one of the PLC beta isozymes, exists as two immunologically distinguishable proteins of 150 (PLC-beta1a) and 140kDa (PLC beta1b). The two isoforms encode in two distinct transcripts and generated by alternative splicing of a single gene. PLC beta1a is preferentially expressed in the cytosol, whereas PLC beta1b is predominantly localized in the nuclei. PLC beta1 is a G-protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5-triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface.

ncbi gi num :

12083581

ncbi acc num :

NP_056007.1

ncbi gb acc num :

NM_015192.3

ncbi mol weight :

150 kDa

ncbi pathways :

AMPK Signaling Pathway (198868); Acetylcholine Regulates Insulin Secretion Pathway (105924); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (161043); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448)

ncbi summary :

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

PLCB1: an enzyme that catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Binds to calmodulin. Uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variant isoforms have been described. Protein type: EC 3.1.4.11; Phospholipase; Carbohydrate Metabolism - inositol phosphate. Chromosomal Location of Human Ortholog: 20p12. Cellular Component: nuclear membrane; cytoplasm; nuclear chromatin; nuclear speck; cytosol; nucleus. Molecular Function: calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; signal transducer activity; protein homodimerization activity; enzyme binding; calcium ion binding; phosphoinositide phospholipase C activity; GTPase activator activity; lamin binding. Biological Process: fat cell differentiation; positive regulation of developmental growth; acetylcholine receptor signaling, muscarinic pathway; inositol phosphate metabolic process; glutamate signaling pathway; metabolic process; positive regulation of interleukin-12 production; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; signal transduction; positive regulation of myoblast differentiation; memory; synaptic transmission; positive regulation of embryonic development; insulin-like growth factor receptor signaling pathway; phosphatidylinositol metabolic process; cerebral cortex development; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of G-protein coupled receptor protein signaling pathway; lipid catabolic process; positive regulation of GTPase activity. Disease: Epileptic Encephalopathy, Early Infantile, 12

size1 :

0.1 mL (Concentrate)

price1 :

180 USD

size2 :

7 mL (RTU)

price2 :

280

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

495