Rabbit Anti-Human Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody | MyBioSource MBS301510 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody

catalog :

MBS301510

quantity :

0.1 mL

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301510

products type :

Antibody

products full name :

Rabbit Anti-Human Placental Alkaline Phosphatase (PLAP) Polyclonal Antibody

products short name :

PLAP

other names :

alkaline phosphatase, placental type preproprotein; Alkaline phosphatase, placental type; alkaline phosphatase, placental type; alkaline phosphatase Regan isozyme; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase 1; alkaline phosphatase, placental; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

products gene name :

PLAP

other gene names :

ALPP; ALPP; ALP; PALP; PLAP; PLAP-1; PLAP; PLAP-1

uniprot entry name :

PPB1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

535

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:40. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:40. IHC Positive Control: Placenta, Seminoma

other info2 :

Immunogen: Recombinant protein encoding human placental alkaline phosphatase. Control Tissue: Placenta, Seminoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 250. Pathway: Cancer Markers

products description :

This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

ncbi gi num :

94721246

ncbi acc num :

NP_001623.3

ncbi gb acc num :

NM_001632.4

ncbi mol weight :

60-70 kDa

ncbi pathways :

Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956); Pyridoxal 5'-phosphate Salvage Pathway (142237)

ncbi summary :

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

uniprot summary :

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]. Protein type: Phosphatase (non-protein); Cell surface; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - folate biosynthesis; EC 3.1.3.1; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q37. Cellular Component: cell surface; plasma membrane; integral to membrane. Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding. Biological Process: dephosphorylation

size1 :

0.1 mL

price1 :

155 USD