product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human p27Kip1 Polyclonal Antibody
catalog :
MBS301470
quantity :
0.1 ml
price :
165 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human, rat, mouse
application :
immunohistochemistry - paraffin section, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS301470
products type :
Antibody
products full name :
Rabbit Anti-Human p27Kip1 Polyclonal Antibody
products short name :
p27Kip1
other names :
cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; p27Kip1
products gene name :
p27Kip1
other gene names :
CDKN1B; CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1; KIP1
uniprot entry name :
CDN1B_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Dog, Hamster, Mouse, Rat
sequence length :
198
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Prostate, Colon
other info2 :
Immunogen: Synthetic peptide derived from C-terminus of human p27Kip1. Control Tissue: Colon, Prostate. Cellular Localization: Nucleus. Entrez Gene Code: 1027. Pathway: Cell Cycle
products description :
Recognizes a protein of 27kDa, identified as p27Kip1. p27Kip1 is a candidate tumor suppressor gene and functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.
ncbi gi num :
4757962
ncbi acc num :
NP_004055.1
ncbi gb acc num :
NM_004064.4
ncbi mol weight :
27 kDa
ncbi pathways :
AKT Phosphorylates Targets In The Cytosol Pathway 106475!!Adaptive Immune System Pathway 366160!!AhR Pathway 755436!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463
ncbi summary :
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
size :
0.1 ml
price :
165 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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