product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Osteopontin Polyclonal Antibody
catalog :
MBS301463
quantity :
0.1 ml
price :
165 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
immunohistochemistry - paraffin section, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS301463
products type :
Antibody
products full name :
Rabbit Anti-Human Osteopontin Polyclonal Antibody
products short name :
Osteopontin
other names :
osteopontin isoform OPN-b; Osteopontin; osteopontin; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); urinary stone protein; uropontin; secreted phosphoprotein 1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
other gene names :
SPP1; SPP1; OPN; BNSP; BSPI; ETA-1; BNSP; OPN; SPP-1
uniprot entry name :
OSTP_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human
sequence length :
300
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Breast Carcinoma
other info2 :
Immunogen: Synthetic peptide derived from internal domain of human osteopontin. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 6696. Pathway: Stem Cell
products description :
Osteopontin is a 34kDa extracellular matrix protein with a cell binding domain. It was originally identified as a major component of the non-collagenous organic bone matrix, however, it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. This antibody should prove useful in studying the expression of this matrix glycoprotein in tissue homeostasis.
ncbi gi num :
4759166
ncbi acc num :
NP_000573.1
ncbi gb acc num :
NM_000582.2
ncbi mol weight :
34 kDa
ncbi pathways :
BDNF Signaling Pathway 712093!!Degradation Of The Extracellular Matrix Pathway 576263!!Direct P53 Effectors Pathway 137939!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!FGF Signaling Pathway 137989!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067
ncbi summary :
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
size :
0.1 ml
price :
165 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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