Mouse Anti-Human MUC-1/EMA/Epithelial Membrane Antigen Monoclonal Antibody (Clone SPM492) | MyBioSource MBS301426 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human MUC-1/EMA/Epithelial Membrane Antigen Monoclonal Antibody (Clone SPM492)

catalog :

MBS301426

quantity :

7 ml

price :

285 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301426

products type :

Antibody

products full name :

Mouse Anti-Human MUC-1/EMA/Epithelial Membrane Antigen Monoclonal Antibody (Clone SPM492)

products short name :

MUC-1/EMA/Epithelial Membrane

other names :

mucin-1 isoform 2; Mucin-1; mucin-1; DF3 antigen; H23 antigen; Medullary cystic kidney disease, autosomal dominant; breast carcinoma-associated antigen DF3; cancer antigen 15-3; carcinoma-associated mucin; episialin; krebs von den Lungen-6; mucin 1, transmembrane; peanut-reactive urinary mucin; polymorphic epithelial mucin; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen; mucin 1, cell surface associated; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT

products gene name :

MUC-1/EMA

other gene names :

MUC1; MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta

uniprot entry name :

MUC1_HUMAN

clonality :

Monoclonal

isotype :

IgG1/k

clone :

SPM492

host :

Mouse

reactivity :

Human

sequence length :

264

form :

7.0 ml pre-diluted mouse monoclonal antibody purified by protein A/G in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:150. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: None Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Breast Carcinoma

other info2 :

Immunogen: Human breast cell line ZR-75-1. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 4582. Pathway: Cancer Markers

products description :

MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cerbB receptors and localize with erbB1 (EGFR) in lactating glands.

ncbi gi num :

67189007

ncbi acc num :

NP_001018016.1

ncbi gb acc num :

NM_001018016.2

ncbi mol weight :

265-400 kDa

ncbi pathways :

IL-7 Signaling Pathway 198857!!Metabolism Of Proteins Pathway 106230!!O-linked Glycosylation Pathway 1127679!!O-linked Glycosylation Of Mucins Pathway 530747!!Post-translational Protein Modification Pathway 161012!!Termination Of O-glycan Biosynthesis Pathway 530748

ncbi summary :

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

size :

7 ml

price :

285 USD

more info or order :

MyBioSource product webpage