Rabbit Anti-Human MMP-2 (72kDa Collagenase IV) Polyclonal Antibody | MyBioSource MBS301413 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human MMP-2 (72kDa Collagenase IV) Polyclonal Antibody

catalog :

MBS301413

quantity :

7 ml

price :

285 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, dog, chicken

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301413

products type :

Antibody

products full name :

Rabbit Anti-Human MMP-2 (72kDa Collagenase IV) Polyclonal Antibody

products short name :

MMP-2

other names :

72 kDa type IV collagenase isoform 2; 72 kDa type IV collagenase; 72 kDa type IV collagenase; collagenase type IV-A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1PEX

products gene name :

MMP-2

other gene names :

MMP2; MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II; CLG4A; MMP-2

uniprot entry name :

MMP2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Dog, Frog, Mouse, Pig, Rabbit, Rat

sequence length :

610

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Placenta, Bladder, Breast, Ovarian Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from near N-terminus of human MMP-2 protein. Control Tissue: Bladder, Breast, Ovarian Carcinoma, Placenta. Cellular Localization: Cytoplasm. Entrez Gene Code: 4313. Pathway: Proteases and Protease Regulators

products description :

MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP-2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids. This antibody reacts with both latent and active MMP-2.

ncbi gi num :

189217853

ncbi acc num :

NP_001121363.1

ncbi gb acc num :

NM_001127891.2

ncbi mol weight :

72 kDa (Pro) and ~66 kDa (Active)

ncbi pathways :

AGE/RAGE Pathway 698754!!ATF-2 Transcription Factor Network Pathway 138006!!Activation Of Matrix Metalloproteinases Pathway 576264!!Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Axon Guidance Pathway 105688!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Collagen Degradation Pathway 730309!!Degradation Of The Extracellular Matrix Pathway 576263!!Developmental Biology Pathway 477129

ncbi summary :

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

size :

7 ml

price :

285 USD