Rabbit Anti-Human MMP-15 Polyclonal Antibody | MyBioSource MBS301410 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human MMP-15 Polyclonal Antibody

catalog :

MBS301410

quantity :

0.1 mL (Concentrate)

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301410

products type :

Antibody

products full name :

Rabbit Anti-Human MMP-15 Polyclonal Antibody

products short name :

MMP-15/MT2-MMP

other names :

matrix metalloproteinase-15 preproprotein; Matrix metalloproteinase-15; matrix metalloproteinase-15; MMP-15; MT-MMP 2; MT2MMP; matrix metalloproteinase 15 (membrane-inserted); membrane-type matrix metalloproteinase 2; membrane-type-2 matrix metalloproteinase; matrix metallopeptidase 15 (membrane-inserted); Membrane-type matrix metalloproteinase 2; MT-MMP 2; MTMMP2; Membrane-type-2 matrix metalloproteinase; MT2-MMP; MT2MMP; SMCP-2

products gene name :

MMP-15/MT2-MMP

other gene names :

MMP15; MMP15; MTMMP2; SMCP-2; MT2-MMP; MMP-15; MT-MMP 2; MTMMP2; MT2-MMP; MT2MMP

uniprot entry name :

MMP15_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

669

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Placenta

other info2 :

Immunogen: Synthetic peptide from the catalytic domain of mouse MMP-15/MT2-MMP protein. Control Tissue: Placenta. Cellular Localization: Cytoplasm. Entrez Gene Code: 4324. Pathway: Proteases and Protease Regulators

products description :

MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively, contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT2-MMP plays a minor role in activation of pro-MMP-2 to its active form in breast carcinomas. Co-expression of MT1-MMP and MT2-MMp in advanced stages of breast carcinomas is indicative of possible involvement of MT2-MMP in its metastasis.

ncbi gi num :

4505211

ncbi acc num :

NP_002419.1

ncbi gb acc num :

NM_002428.2

ncbi mol weight :

62 and 66 kDa

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family. Protein type: EC 3.4.24.-; Protease; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: extracellular matrix; integral to plasma membrane; plasma membrane. Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding. Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; protein modification process; proteolysis; response to estradiol stimulus

size1 :

0.1 mL (Concentrate)

price1 :

165 USD

size2 :

7 mL (RTU)

price2 :

265

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

420