Rabbit Anti-Human MMP-14/MT1-MMP Polyclonal Antibody | MyBioSource MBS301409 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human MMP-14/MT1-MMP Polyclonal Antibody

catalog :

MBS301409

quantity :

0.1 ml

price :

165 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat, human, mouse

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301409

products type :

Antibody

products full name :

Rabbit Anti-Human MMP-14/MT1-MMP Polyclonal Antibody

products short name :

MMP-14/MT1-MMP

other names :

matrix metalloproteinase-14 preproprotein; Matrix metalloproteinase-14; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase; matrix metallopeptidase 14 (membrane-inserted); MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP

products gene name :

MMP-14/MT1-MMP

other gene names :

MMP14; MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

uniprot entry name :

MMP14_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Hamster, Mouse, Pig, Rabbit, Rat

sequence length :

582

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:30. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:30. IHC Positive Control: Placenta, Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from internal region of human MMP-14 protein. Control Tissue: Breast Carcinoma, Placenta. Cellular Localization: Cytoplasm. Entrez Gene Code: 4323. Pathway: Proteases and Protease Regulators

products description :

MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of the matrix metalloproteinase family, ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively, contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT1-MMP gets activated through the membrane plasmin cascade. It binds to TIMP-2 first and then to MMP-2 forming a trimer. It cleaves the pro-MMP-2 to its active form.

ncbi gi num :

4826834

ncbi acc num :

NP_004986.1

ncbi gb acc num :

NM_004995.3

ncbi mol weight :

~66 kDa (Latent) and ~54 kDa (Active)

ncbi pathways :

AGE/RAGE Pathway 698754!!Activation Of Matrix Metalloproteinases Pathway 576264!!Collagen Degradation Pathway 730309!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262!!GnRH Signaling Pathway 83091!!GnRH Signaling Pathway 502!!HIF-2-alpha Transcription Factor Network Pathway 137956!!Matrix Metalloproteinases Pathway 198900!!Senescence And Autophagy Pathway 198780

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

size :

0.1 ml

price :

165 USD

more info or order :

MyBioSource product webpage