catalog number :
MBS301387
products full name :
Rabbit Anti-Human MART-1/MELAN-A Polyclonal Antibody
products short name :
MART-1/Melan-A
other names :
melanoma antigen recognized by T-cells 1; Melanoma antigen recognized by T-cells 1; melanoma antigen recognized by T-cells 1; antigen LB39-AA; antigen SK29-AA; protein Melan-A; melan-A; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A
products gene name :
MART-1/Melan-A
other gene names :
MLANA; MLANA; MART1; MART-1; MART1; MART-1
uniprot entry name :
MAR1_HUMAN
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Melanoma
other info2 :
Immunogen: Recombinant human MART-1 protein. Control Tissue: Melanoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 2315. Pathway: Cancer Markers
products description :
MART-1 or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-1, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
ncbi acc num :
NP_005502.1
ncbi gb acc num :
NM_005511.1