product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Keratin 5 Polyclonal Antibody
catalog :
MBS301358
quantity :
0.1 ml
price :
170 USD
clonality :
polyclonal
host :
rabbit
reactivity :
rat, dog, mouse, human
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS301358
products type :
Antibody
products full name :
Rabbit Anti-Human Keratin 5 Polyclonal Antibody
products short name :
Keratin 5
other names :
keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 5; 58 kda cytokeratin; CK-5; cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); type-II keratin Kb5; keratin 5, type II; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
other gene names :
KRT5; KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A; CK-5; K5
uniprot entry name :
K2C5_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rat
sequence length :
590
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Skin, Breast, Prostate, Mesothelioma
other info2 :
Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 5. Control Tissue: Breast, Mesothelioma, Prostate, Skin. Cellular Localization: Cytoplasm. Entrez Gene Code: 3852. Pathway: Stem Cell
products description :
Twenty human keratins are divided into acidic (pI 6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor identification. Point mutations in cytokeratin 5 gene can cause various types of epidermolysis bullosa simplex. It is expressed in breast myoepithelia cells, prostate basal cells, most epithelial and biphasic mesotheliomas.
ncbi gi num :
119395754
ncbi acc num :
NP_000415.2
ncbi gb acc num :
NM_000424.3
ncbi mol weight :
58 kDa
ncbi pathways :
Glucocorticoid Receptor Regulatory Network Pathway 138014
ncbi summary :
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
size :
0.1 ml
price :
170 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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