Rabbit Anti-Human Keratin 14 Polyclonal Antibody | MyBioSource MBS301350 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Keratin 14 Polyclonal Antibody

catalog :

MBS301350

quantity :

0.1 mL (Concentrate)

price :

155 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301350

products type :

Antibody

products full name :

Rabbit Anti-Human Keratin 14 Polyclonal Antibody

products short name :

Keratin 14

other names :

keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; Cytokeratin-14; CK-14; Keratin-14; K14

other gene names :

KRT14; KRT14; K14; NFJ; CK14; EBS3; EBS4; CK-14; K14

uniprot entry name :

K1C14_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Mouse, Pig, Rat

sequence length :

472

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Staining of formalin-fixed tissues requires digesting tissue sections with Pepsin for 10 min at 37 degree C. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Skin, Squamous Cell Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from C-terminus of human cytokeratin 14. Control Tissue: Skin, Squamous Cell Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 3861. Pathway: Stem Cell

products description :

Cytokeratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 5.

ncbi gi num :

15431310

ncbi acc num :

NP_000517.2

ncbi gb acc num :

NM_000526.4

ncbi mol weight :

50 kDa

ncbi pathways :

Corticotropin-releasing Hormone Pathway (920957); Glucocorticoid Receptor Regulatory Network Pathway (138014)

ncbi summary :

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

uniprot summary :

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: keratin filament; cytoplasm; intermediate filament; cytosol; nucleus. Molecular Function: protein binding; structural constituent of cytoskeleton. Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; intermediate filament bundle assembly; response to ionizing radiation; aging; hair cycle. Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type

size1 :

0.1 mL (Concentrate)

price1 :

155 USD

size2 :

7 mL (RTU)

price2 :

225

size3 :

0.5 mL (Concentrate)

price3 :

275

size4 :

1 mL (Concentrate)

price4 :

385