Rabbit Anti-Human HSP70 Polyclonal Antibody | MyBioSource MBS301300 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human HSP70 Polyclonal Antibody

catalog :

MBS301300

quantity :

0.1 mL (Concentrate)

price :

180 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301300

products type :

Antibody

products full name :

Rabbit Anti-Human HSP70 Polyclonal Antibody

products short name :

Heat Shock Protein 70

other names :

heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; heat shock 70 kDa protein 1/2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70kDa protein 1A; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

products gene name :

HSP70

other gene names :

HSPA1A; HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103; HSPA1; HSX70; HSP70-1/HSP70-2; HSP70.1/HSP70.2

uniprot entry name :

HSP71_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Mouse, Pig, Rat

sequence length :

641

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Breast Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: 293T Cell Lysate

other info2 :

Immunogen: Synthetic peptide corresponding to aa 574-591 of human HSP70 protein. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 3303. Pathway: T Cell Markers

products description :

The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, they participate in the translocation of polypeptides across cell membranes and to the nucleus, and they aid in the proper folding of nascent polypeptide chain. HSP70 expression is strongly induced in response to heat stress.

ncbi gi num :

194248072

ncbi acc num :

NP_005336.3

ncbi gb acc num :

NM_005345.5

ncbi mol weight :

70 kDa

ncbi pathways :

AUF1 (hnRNP D0) Destabilizes MRNA Pathway (187212); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Apoptosis Modulation By HSP70 Pathway (198877); Attenuation Phase Pathway (1127556); Cellular Response To Heat Stress Pathway (1127553); Cellular Responses To Stress Pathway (645258); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); Endocytosis Pathway (102279)

ncbi summary :

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

uniprot summary :

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70. Protein type: Motility/polarity/chemotaxis; Chaperone; Heat shock protein. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: signalosome; focal adhesion; mitochondrion; endoplasmic reticulum; inclusion body; cytosol; ribonucleoprotein complex; centriole; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nuclear speck; nucleus; ubiquitin ligase complex; vesicle. Molecular Function: viral receptor activity; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; heat shock protein binding; unfolded protein binding; double-stranded RNA binding; ATPase activity; protein N-terminus binding; ATPase activity, coupled; ATP binding. Biological Process: negative regulation of cell proliferation; entry of virus into host cell; protein stabilization; positive regulation of erythrocyte differentiation; mRNA catabolic process; protein refolding; gene expression; negative regulation of protein ubiquitination; negative regulation of cell growth; response to unfolded protein; negative regulation of apoptosis

size1 :

0.1 mL (Concentrate)

price1 :

180 USD

size2 :

7 mL (RTU)

price2 :

280

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

495