Rabbit Anti-Human HDAC-1 Polyclonal Antibody | MyBioSource MBS301297 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human HDAC-1 Polyclonal Antibody

catalog :

MBS301297

quantity :

0.1 mL (Concentrate)

price :

165 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301297

products type :

Antibody

products full name :

Rabbit Anti-Human HDAC-1 Polyclonal Antibody

products short name :

HDAC1

other names :

histone deacetylase 1; Histone deacetylase 1; histone deacetylase 1; reduced potassium dependency, yeast homolog-like 1; histone deacetylase 1

products gene name :

HDAC1

other gene names :

HDAC1; HDAC1; HD1; RPD3; GON-10; RPD3L1; RPD3L1; HD1

uniprot entry name :

HDAC1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Dog, Mouse, Pig, Rabbit, Rat

sequence length :

482

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Tonsil, Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from C-terminus of human HDAC1 protein. Control Tissue: Breast Carcinoma, Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 3065. Pathway: Transcriptional Regulators

products description :

Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.

ncbi gi num :

13128860

ncbi acc num :

NP_004955.2

ncbi gb acc num :

NM_004964.2

ncbi mol weight :

58 kDa

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1127620); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1127618); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Androgen Receptor Signaling Pathway (198806)

ncbi summary :

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

uniprot summary :

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Protein type: EC 3.5.1.98; Hydrolase; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 1p34. Cellular Component: nucleoplasm; protein complex; Sin3 complex; nuclear chromatin; cytoplasm; histone deacetylase complex; NuRD complex; chromatin; nucleus; cytosol. Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity. Biological Process: establishment and/or maintenance of chromatin architecture; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; mitotic cell cycle; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis

size1 :

0.1 mL (Concentrate)

price1 :

165 USD

size2 :

7 mL (RTU)

price2 :

225

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

420