antibody

Rabbit Anti-Human GluR2/3 Polyclonal Antibody

MBS301280

0.1 mL (Concentrate)

180 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Rabbit Anti-Human GluR2/3 Polyclonal Antibody

GluR2/3

metabotropic glutamate receptor 3; Metabotropic glutamate receptor 3; metabotropic glutamate receptor 3; glutamate metabotropic receptor 3; glutamate receptor, metabotropic 3

GluR2/3

GRM3; GRM3; GLUR3; mGlu3; GPRC1C; MGLUR3; GPRC1C; MGLUR3; mGluR3

GRM3_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Monkey, Mouse, Rat

879

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Cerebellum

Immunogen: Synthetic peptide from C-terminus of human GluR2/3. Control Tissue: Cerebellum. Cellular Localization: Cytoplasm. Entrez Gene Code: 2913. Pathway: Neuroscience

Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors, which when activated can activate phopholipases or adenylylcyclase, depending on the neuron.

46358417

NP_000831.2

NM_000840.2

~105 kDa

Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (106381); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198839); Glutamatergic Synapse Pathway (213818)

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq, Jul 2008]

mGluR3: metabotropic glutamate receptor 3, a G-protein coupled receptor for glutamate. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 3; Receptor, GPCR. Chromosomal Location of Human Ortholog: 7q21.1-q21.2. Cellular Component: presynaptic membrane; postsynaptic membrane; axon; integral to plasma membrane; postsynaptic density; dendritic spine; plasma membrane. Molecular Function: G-protein coupled receptor activity; group II metabotropic glutamate receptor activity; group III metabotropic glutamate receptor activity; calcium channel regulator activity; glutamate receptor activity. Biological Process: synaptic transmission; regulation of synaptic transmission, glutamatergic; negative regulation of adenylate cyclase activity; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway

0.1 mL (Concentrate)

180 USD

7 mL (RTU)

265

0.5 mL (Concentrate)

330

1 mL (Concentrate)

495