catalog number :
MBS301275
products full name :
Mouse Anti-Human GFAP Monoclonal Antibody (Clone SPM507)
products short name :
Glial Fibrillary Acidic Protein
other names :
glial fibrillary acidic protein isoform 2; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein
products gene name :
GFAP
other gene names :
GFAP; GFAP; GFAP
uniprot entry name :
GFAP_HUMAN
reactivity :
Human. Predicted Reactivity: Bovine, Dog, Mouse, Rabbit, Rat
form :
0.1 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: None Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Brain, Astrocytoma
other info2 :
Immunogen: Porcupine spinal cord. Control Tissue: Astrocytoma, Brain. Cellular Localization: Cytoplasm. Entrez Gene Code: 2670. Pathway: Stem Cell
products description :
Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system. The antibody to GFAP is useful for documenting astrocytic differentiation in tumors outside the CNS.
ncbi acc num :
NP_001124491.1
ncbi gb acc num :
NM_001131019.2
ncbi mol weight :
51-52 kDa
ncbi pathways :
Neural Crest Differentiation Pathway 672460!!Nuclear Signaling By ERBB4 Pathway 530744!!Signal Transduction Pathway 477114!!Signaling By ERBB4 Pathway 530741!!Spinal Cord Injury Pathway 739007
ncbi summary :
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]