catalog number :
MBS301201
products full name :
Rabbit Anti-Human DOG-1 Polyclonal Antibody
products short name :
DOG-1
other names :
anoctamin-1; Anoctamin-1; anoctamin-1; Ca2+-activated Cl- channel; discovered on gastrointestinal stromal tumors protein 1; oral cancer overexpressed 2; transmembrane protein 16A (eight membrane-spanning domains); tumor-amplified and overexpressed sequence 2; anoctamin 1, calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2
products gene name :
DOG-1
other gene names :
ANO1; ANO1; DOG1; TAOS2; ORAOV2; TMEM16A; DOG1; ORAOV2; TAOS2; TMEM16A
uniprot entry name :
ANO1_HUMAN
reactivity :
Human. Predicted Reactivity: Dog, Frog, Mouse, Rabbit, Rat
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Gastrointestinal Stromal Tumor
other info2 :
Immunogen: Synthetic peptide of human DOG-1 protein. Control Tissue: Gastrointestinal Stromal Tumor. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 55107. Pathway: Oncogenesis
products description :
DOG-1, a novel marker, is expressed ubiquitously in gastrointestinal stromal tumors (GIST) irrespective of KIT or PDGFR alpha mutation status.
ncbi acc num :
NP_060513.5
ncbi gb acc num :
NM_018043.5
ncbi mol weight :
~78 kDa
ncbi pathways :
Ion Channel Transport Pathway 187193!!Orphan Transporters Pathway 1127546!!Stimuli-sensing Channels Pathway 771568!!Transmembrane Transport Of Small Molecules Pathway 106572