Rabbit Anti-Human Connexin 43 Polyclonal Antibody | MyBioSource MBS301169 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Connexin 43 Polyclonal Antibody

catalog :

MBS301169

quantity :

0.1 ml

price :

170 USD

clonality :

polyclonal

host :

rabbit

reactivity :

dog, rat, human, mouse

application :

immunohistochemistry - paraffin section, western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS301169

products type :

Antibody

products full name :

Rabbit Anti-Human Connexin 43 Polyclonal Antibody

products short name :

Connexin-43

other names :

gap junction alpha-1 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction protein, alpha 1, 43kDa; Connexin-43; Cx43; Gap junction 43 kDa heart protein

other gene names :

GJA1; GJA1; HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1; GJAL; Cx43

uniprot entry name :

CXA1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat

sequence length :

382

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Heart. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: MCF7 Cell Lysate

other info2 :

Immunogen: Synthetic peptide derived from a segment of the 3rd cytoplasmic domain of rat connexin-43. Control Tissue: Heart. Cellular Localization: Cytoplasm. Entrez Gene Code: 2697. Pathway: Cell Junctions

products description :

The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Connexins are also thought to be essential for the development of labor contractions.

ncbi gi num :

4504001

ncbi acc num :

NP_000156.1

ncbi gb acc num :

NM_000165.4

ncbi mol weight :

43 kDa

ncbi pathways :

Arrhythmogenic Right Ventricular Cardiomyopathy Pathway 672454!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Corticotropin-releasing Hormone Pathway 920957!!EGFR1 Signaling Pathway 198782!!Formation Of Annular Gap Junctions Pathway 105934!!Gap Junction Pathway 83072!!Gap Junction Pathway 483!!Gap Junction Assembly Pathway 105928

ncbi summary :

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]

size :

0.1 ml

price :

170 USD

more info or order :

MyBioSource product webpage