Mouse Anti-Human CD74 Monoclonal Antibody (Clone SPM523) | MyBioSource MBS301106 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human CD74 Monoclonal Antibody (Clone SPM523)

catalog :

MBS301106

quantity :

0.1 mL (Concentrate)

price :

180 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SPM523

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301106

products type :

Antibody

products full name :

Mouse Anti-Human CD74 Monoclonal Antibody (Clone SPM523)

products short name :

CD74

other names :

HLA class II histocompatibility antigen gamma chain isoform c; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen gamma chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia-associated invariant chain; MHC HLA-DR gamma chain; gamma chain of class II antigens; p33; CD74 molecule, major histocompatibility complex, class II invariant chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD_antigen: CD74

products gene name :

CD74

other gene names :

CD74; CD74; II; DHLAG; HLADG; Ia-GAMMA; DHLAG; Ii

uniprot entry name :

HG2A_HUMAN

clonality :

Monoclonal

isotype :

IgG1/k

clone :

SPM523

host :

Mouse

reactivity :

Human

sequence length :

160

form :

0.1 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Ramos Cell Lysate

other info2 :

Immunogen: Recombinant human CD74 protein. Control Tissue: Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 972. Pathway: Lymphocyte Signaling

products description :

Expression of the invariant chain of the HLA-DR antigen (CD74) by B-lymphocytes begins in the pre-B stage but lost before the final differentiation to plasma cells. This antibody is expressed in Blymphocytes and many B-cell lymphomas.

ncbi gi num :

68448537

ncbi acc num :

NP_001020329.1

ncbi gb acc num :

NM_001025158.2

ncbi mol weight :

35 kDa

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Immune System Pathway (106386); MHC Class II Antigen Presentation Pathway (645290); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

uniprot summary :

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Endoplasmic reticulum; Receptor, cytokine. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: Golgi membrane; multivesicular body; lysosomal lumen; cell surface; membrane; lysosomal membrane; integral to membrane; plasma membrane; vacuole; trans-Golgi network membrane; intracellular; external side of plasma membrane; MHC class II protein complex. Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; beta-amyloid binding; cytokine binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; MHC class II protein binding. Biological Process: activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; prostaglandin biosynthetic process; signal transduction; negative regulation of T cell differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; intracellular protein transport; positive regulation of fibroblast proliferation; negative regulation of mature B cell apoptosis; positive regulation of B cell proliferation; protein complex assembly; positive thymic T cell selection; negative regulation of peptide secretion; positive regulation of T-helper 2 type immune response; chaperone cofactor-dependent protein folding; regulation of macrophage activation; T cell selection; negative thymic T cell selection; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; immunoglobulin mediated immune response; positive regulation of cytokine and chemokine mediated signaling pathway; antigen processing and presentation of endogenous antigen; negative regulation of apoptosis

size1 :

0.1 mL (Concentrate)

price1 :

180 USD

size2 :

7 mL (RTU)

price2 :

280

size3 :

0.5 mL (Concentrate)

price3 :

310

size4 :

1 mL (Concentrate)

price4 :

465