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antibody

Rabbit Anti-Human CREB-Binding Protein (CBP) Polyclonal Antibody

MBS301023

0.1 ml

170 USD

polyclonal

rabbit

human

immunohistochemistry - paraffin section, immunohistochemistry

MBS301023

Antibody

Rabbit Anti-Human CREB-Binding Protein (CBP) Polyclonal Antibody

CREB-Binding Protein

CREB-binding protein isoform b; CREB-binding protein; CREB-binding protein; CREB binding protein; N/A

CBP

CREBBP; CREBBP; CBP; RSTS; KAT3A; CBP

CBP_HUMAN

Polyclonal

IgG

Rabbit

Human

2404

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil

Immunogen: Synthetic peptide corresponding to aa 2422-2441 of human CBP. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 1387. Pathway: Transcriptional Regulators

CBP for CREB-binding protein is a 265kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

119943102

NP_001073315.1

NM_001079846.1

265 kDa

Activation Of Gene Expression By SREBF (SREBP) Pathway 685552!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Androgen Receptor Signaling Pathway 198806!!Angiogenesis Pathway 198772!!Attenuation Phase Pathway 1127556!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 477138!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

0.1 ml

170 USD