Mouse Anti-Human Cadherin-E/E-Cadherin Monoclonal Antibody (Clone SPM471) | MyBioSource MBS300988 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Cadherin-E/E-Cadherin Monoclonal Antibody (Clone SPM471)

catalog :

MBS300988

quantity :

0.1 mL (Concentrate)

price :

180 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SPM471

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300988

products type :

Antibody

products full name :

Mouse Anti-Human Cadherin-E/E-Cadherin Monoclonal Antibody (Clone SPM471)

products short name :

Cadherin-E/E-Cadherin

other names :

cadherin-1 preproprotein; Cadherin-1; cadherin-1; CAM 120/80; E-Cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; cell-CAM 120/80; epithelial cadherin; uvomorulin; cadherin 1, type 1, E-cadherin (epithelial); CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD_antigen: CD324Cleaved into the following 3 chains:E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3

other gene names :

CDH1; CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324; CDHE; UVO; E-cadherin

uniprot entry name :

CADH1_HUMAN

clonality :

Monoclonal

isotype :

IgG2b/k

clone :

SPM471

host :

Mouse

reactivity :

Human

sequence length :

882

form :

0.1 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 30 min at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Breast, Tonsil, Skin

other info2 :

Immunogen: Recombinant protein encoding aa 600-707 of human cadherin-E. Control Tissue: Breast, Skin, Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 999. Pathway: Stem Cell

products description :

E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity.

ncbi gi num :

4757960

ncbi acc num :

NP_004351.1

ncbi gb acc num :

NM_004360.3

ncbi mol weight :

120 kDa

ncbi pathways :

Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Adherens Junctions Interactions Pathway (119533); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (105680); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Arf6 Trafficking Events Pathway (137954); Bacterial Invasion Of Epithelial Cells Pathway (149807)

ncbi summary :

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

uniprot summary :

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Ubiquitin ligase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: apical junction complex; internal side of plasma membrane; focal adhesion; cell surface; basolateral plasma membrane; lateral loop; extracellular region; integral to membrane; catenin complex; trans-Golgi network; actin cytoskeleton; cell-cell adherens junction; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nerve terminal; cell junction; endosome; lateral plasma membrane. Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; beta-catenin binding; GTPase activating protein binding; calcium ion binding; cell adhesion molecule binding; ankyrin binding; glycoprotein binding; protein phosphatase binding. Biological Process: response to drug; intercellular junction assembly and maintenance; regulation of immune response; extracellular matrix organization and biogenesis; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; regulation of caspase activity; trophectodermal cell differentiation; regulation of water loss via skin; extracellular matrix disassembly; cell-cell adhesion; sensory perception of sound; synaptogenesis; pituitary gland development; protein metabolic process; calcium-dependent cell-cell adhesion; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; homophilic cell adhesion; protein homooligomerization; neurite development; negative regulation of epithelial cell proliferation; cell structure disassembly during apoptosis. Disease: Gastric Cancer, Hereditary Diffuse; Breast Cancer; Prostate Cancer; Ovarian Cancer; Endometrial Cancer

size1 :

0.1 mL (Concentrate)

price1 :

180 USD

size2 :

7 mL (RTU)

price2 :

280

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

475