Rabbit Anti-Human AKT-1/PKB (phospho S473) Polyclonal Antibody | MyBioSource MBS300935 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human AKT-1/PKB (phospho S473) Polyclonal Antibody

catalog :

MBS300935

quantity :

0.1 ml

price :

170 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat, mouse

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS300935

products type :

Antibody

products full name :

Rabbit Anti-Human AKT-1/PKB (phospho S473) Polyclonal Antibody

products short name :

AKT-1/PKB

other names :

RAC-alpha serine/threonine-protein kinase; RAC-alpha serine/threonine-protein kinase; RAC-alpha serine/threonine-protein kinase; AKT1m; PKB alpha; RAC-PK-alpha; protein kinase B alpha; proto-oncogene c-Akt; rac protein kinase alpha; v-akt murine thymoma viral oncogene homolog 1; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

products gene name :

AKT-1/PKB

other gene names :

AKT1; AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; PKB; RAC; PKB; PKB alpha

uniprot entry name :

AKT1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Frog, Mouse, Rat

sequence length :

480

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:25. IHC Positive Control: Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide derived from the region of human AKT-1 that contains phosphorylated site of serine 473. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 207. Pathway: AKT/PKB Signaling

products description :

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. The antibody labels activated AKT-1 that is phosphorylated at 473 serine residue. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).

ncbi gi num :

62241013

ncbi acc num :

NP_001014431.1

ncbi gb acc num :

NM_001014431.1

ncbi mol weight :

60 kDa

ncbi pathways :

AGE/RAGE Pathway 698754!!AKT Phosphorylates Targets In The Cytosol Pathway 106475!!AKT Phosphorylates Targets In The Nucleus Pathway 106476!!AKT-mediated Inactivation Of FOXO1A Pathway 106333!!AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Acute Myeloid Leukemia Pathway 83117

ncbi summary :

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

size :

0.1 ml

price :

170 USD

more info or order :

MyBioSource product webpage