Rabbit Anti-Human Oct-3/Oct-4 Polyclonal Antibody | MyBioSource MBS300915 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Oct-3/Oct-4 Polyclonal Antibody

catalog :

MBS300915

quantity :

0.1 ml

price :

170 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, dog

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300915

products type :

Antibody

products full name :

Rabbit Anti-Human Oct-3/Oct-4 Polyclonal Antibody

products short name :

OCT-3/OCT-4

other names :

POU domain, class 5, transcription factor 1 isoform 2; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 1; POU domain transcription factor OCT4; POU-type homeodomain-containing DNA-binding protein; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; POU class 5 homeobox 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3

products gene name :

OCT-3/OCT-4

other gene names :

POU5F1; POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3

uniprot entry name :

PO5F1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Pig, Rabbit

sequence length :

190

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Seminoma

other info2 :

Immunogen: Synthetic peptide corresponding to internal region of human OCT-3/OCT-4 protein. Control Tissue: Seminoma. Cellular Localization: Nucleus. Entrez Gene Code: 5460. Pathway: Stem Cell

products description :

Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem cell renewal, and up or down regulation induce divergent developmental programs.

ncbi gi num :

291167757

ncbi acc num :

NP_001167002.1

ncbi gb acc num :

NM_001173531.2

ncbi mol weight :

~43 kDa

ncbi pathways :

Cardiac Progenitor Differentiation Pathway 712094!!Developmental Biology Pathway 477129!!HIF-2-alpha Transcription Factor Network Pathway 137956!!POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway 1127697!!POU5F1 (OCT4), SOX2, NANOG Repress Genes Related To Differentiation Pathway 1127698!!SIDS Susceptibility Pathways 198901!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1026136!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1033502!!Transcriptional Regulation Of Pluripotent Stem Cells Pathway 1127696!!Wnt Signaling Pathway And Pluripotency 198847

ncbi summary :

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

size :

0.1 ml

price :

170 USD