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antibody

Mouse Anti-Human CD63 Monoclonal Antibody (Clone SPM524)

MBS300876

7 ml

285 USD

monoclonal

mouse

human

immunohistochemistry, immunohistochemistry - paraffin section

MBS300876

Antibody

Mouse Anti-Human CD63 Monoclonal Antibody (Clone SPM524)

CD63

CD63 antigen isoform A; CD63 antigen; CD63 antigen; CD63 antigen (melanoma 1 antigen); granulophysin; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; ocular melanoma-associated antigen; tetraspanin-30; tspan-30; CD63 molecule; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD_antigen: CD63

CD63

CD63; CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30; MLA1; TSPAN30; LAMP-3; Tspan-30

CD63_HUMAN

Monoclonal

IgG1/k

SPM524

Mouse

Human

238

7.0 ml pre-diluted mouse monoclonal antibody purified by protein A/G in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Melanoma

Immunogen: Recombinant human CD63 protein. Control Tissue: Melanoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 967. Pathway: Hematopoiesis

CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40.

383872447

NP_001244318.1

NM_001257389.1

53 kDa

Hemostasis Pathway 106028!!Lysosome Pathway 99052!!Lysosome Pathway 96865!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

7 ml

285 USD