product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Actin-beta Polyclonal Antibody
catalog :
MBS300767
quantity :
1 ml
price :
455 USD
clonality :
polyclonal
host :
rabbit
reactivity :
mouse, chicken, rat, human, dog
application :
immunohistochemistry - paraffin section, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS300767
products type :
Antibody
products full name :
Rabbit Anti-Human Actin-beta Polyclonal Antibody
products short name :
Actin-beta
other names :
actin, cytoplasmic 1; Actin, cytoplasmic 1; actin, cytoplasmic 1; PS1TP5-binding protein 1; beta cytoskeletal actin; actin, beta; Beta-actinActin, cytoplasmic 1, N-terminally processed
other gene names :
ACTB; ACTB; BRWS1; PS1TP5BP1; N/A
uniprot entry name :
ACTB_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Bovine, Chicken, Dog, Frog, Mouse, Pig, Rabbit, Rat
sequence length :
375
form :
1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Heart, Leiomyoma, Skin
other info2 :
Immunogen: N-terminus of human beta actin. Control Tissue: Heart, Leiomyoma, Skin. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 60. Pathway: Cytoskeletal Proteins
products description :
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three, alpha-actin (skeletal, cardiac, and smooth muscle), one beta-actin (beta-nonmuscle), and two gemma- actins (gemma-smooth nuscle and gemma-nonmuscle). Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
ncbi gi num :
4501885
ncbi acc num :
NP_001092.1
ncbi gb acc num :
NM_001101.3
ncbi mol weight :
42 kDa
ncbi pathways :
Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Arrhythmogenic Right Ventricular Cardiomyopathy Pathway 672454!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Axon Guidance Pathway 105688!!Bacterial Invasion Of Epithelial Cells Pathway 149807!!Bacterial Invasion Of Epithelial Cells Pathway 148661!!Chaperonin-mediated Protein Folding Pathway 106242!!Chromatin Modifying Enzymes Pathway 1127682
ncbi summary :
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
size :
1 ml
price :
455 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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