Rabbit Anti-Human Cholecystokinin (26-33) (CCK-8) Polyclonal Antibody | MyBioSource MBS300760 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Cholecystokinin (26-33) (CCK-8) Polyclonal Antibody

catalog :

MBS300760

quantity :

7 ml

price :

265 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, dog, chicken

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300760

products type :

Antibody

products full name :

Rabbit Anti-Human Cholecystokinin (26-33) (CCK-8) Polyclonal Antibody

products short name :

Cholecystokinin

other names :

cholecystokinin preproprotein; Cholecystokinin; cholecystokinin; cholecystokinin triacontatriapeptide; prepro-cholecystokinin; cholecystokinin; (1-49)-CCK58

products gene name :

CCK-8

other gene names :

CCK; CCK; CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5

uniprot entry name :

CCKN_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Dog, Frog, Mouse, Pig, Rat

sequence length :

115

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. (For IHC only)

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blotting (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Stomach. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Brain Cell Lysate

other info2 :

Immunogen: Synthetic peptide sulfated cholecystokinin (26-33) amide (sulfated CCK-8). Control Tissue: Stomach. Cellular Localization: Cytoplasm. Entrez Gene Code: 885. Pathway: Hormones

products description :

Cholecystokinin (CCK) is a neuropeptide hormone and neurotransmitter widely distributed throughout the gastrointestinal (GI) tract and the central nervous system (CNS). Antibodies that react specifically with CCK are useful for the study of the mode of action, differential tissue expression and intracellular and subcellular localization of CCK in the CNS and periphery as well as in neuroendocrine cells of the digestive system.

ncbi gi num :

4502605

ncbi acc num :

NP_000720.1

ncbi gb acc num :

NM_000729.4

ncbi mol weight :

12 kDa

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Disease Pathway 530764!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Metabolic Disorders Of Biological Oxidation Enzymes Pathway 1127637!!Peptide Ligand-binding Receptors Pathway 106358!!Signal Transduction Pathway 477114

ncbi summary :

Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

size :

7 ml

price :

265 USD