catalog number :
MBS300727
products full name :
Rabbit Anti-Human OCT-2 Polyclonal Antibody
products short name :
2-Oct
other names :
POU domain, class 2, transcription factor 2 isoform 1; POU domain, class 2, transcription factor 2; POU domain, class 2, transcription factor 2; OTF-2; homeobox protein; lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; octamer-binding protein 2; octamer-binding transcription factor 2; POU class 2 homeobox 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2
products gene name :
2-Oct
other gene names :
POU2F2; POU2F2; OCT2; OTF2; Oct-2; OCT2; OTF2; Oct-2; OTF-2
uniprot entry name :
PO2F2_HUMAN
reactivity :
Human. Predicted Reactivity: Dog, Mouse, Pig, Rabbit
form :
1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Tonsil
other info2 :
Immunogen: Synthetic peptide derived from C-terminus of human OCT-2. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 5452. Pathway: Signal Transduction
products description :
Oct-2 is a transcription factor belonging to the POU homeo-domain family that binds to the Ig gene octomer sites regulating B cell specific genes. Oct-2 protein expression is not restricted to B cells, although expression levels are much higher in these cells. Oct-2 expression is reported to be significantly greater in germinal center derived lymphomas, although other B cell lymphomas also display high levels of expression.
ncbi acc num :
NP_001193954.1
ncbi gb acc num :
NM_001207025.2
ncbi mol weight :
~80 kDa
ncbi pathways :
BCR Signaling Pathway 138058!!SIDS Susceptibility Pathways 198901
ncbi summary :
The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]