Rabbit Anti-Human PARP (Poly ADP-Ribose Polymerase) Polyclonal Antibody | MyBioSource MBS300716 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human PARP (Poly ADP-Ribose Polymerase) Polyclonal Antibody

catalog :

MBS300716

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, chicken

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300716

products type :

Antibody

products full name :

Rabbit Anti-Human PARP (Poly ADP-Ribose Polymerase) Polyclonal Antibody

products short name :

Poly ADP-Ribose Polymerase

other names :

poly; Poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; poly (ADP-ribose) polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1

products gene name :

PARP

other gene names :

PARP1; PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1

uniprot entry name :

PARP1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Frog, Hamster, Mouse, Rat

sequence length :

1014

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Tonsil

other info2 :

Immunogen: Synthetic peptide derived from N-terminus of human PARP protein. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 142. Pathway: Apoptosis

products description :

PARP uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death and is mediated by the caspase CPP32 protein.

ncbi gi num :

156523968

ncbi acc num :

NP_001609.2

ncbi gb acc num :

NM_001618.3

ncbi mol weight :

116 kDa

ncbi pathways :

BER Complex Pathway 413429!!BER Complex Pathway 468377!!Base Excision Repair Pathway 83043!!Base Excision Repair Pathway 451!!Caspase Cascade In Apoptosis Pathway 137974!!Corticotropin-releasing Hormone Pathway 920957!!Disease Pathway 530764!!Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway 645266!!FAS Pathway And Stress Induction Of HSP Regulation 198894!!Gene Expression Pathway 105937

ncbi summary :

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

size :

7 ml

price :

220 USD