Rabbit Anti-Human Bcl-xL Polyclonal Antibody | MyBioSource MBS300712 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Bcl-xL Polyclonal Antibody

catalog :

MBS300712

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300712

products type :

Antibody

products full name :

Rabbit Anti-Human Bcl-xL Polyclonal Antibody

products short name :

BCL-XL

other names :

bcl-2-like protein 1 isoform 2; Bcl-2-like protein 1; bcl-2-like protein 1; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52; BCL2-like 1; Apoptosis regulator Bcl-X

products gene name :

BCL-XL

other gene names :

BCL2L1; BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S; BCL2L; BCLX; Bcl2-L-1

uniprot entry name :

B2CL1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Mouse, Rat

sequence length :

170

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. (For IHC only)

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blotting (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Colon Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: 293T Cell Lysate

other info2 :

Immunogen: Synthetic peptide derived from N-terminus of human BCL-X protein. Control Tissue: Colon Carcinoma, Hodgkin's Lymphoma. Cellular Localization: Cytoplasm, Membrane, Nucleus. Entrez Gene Code: 598. Pathway: Apoptosis

products description :

Bcl-X has two isoforms, Bcl-XL (long) a 241 amino acid protein and Bcl-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family

ncbi gi num :

4502381

ncbi acc num :

NP_001182.1

ncbi gb acc num :

NM_001191.2

ncbi mol weight :

27 kDa

ncbi pathways :

Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822!!BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway 105664!!Chronic Myeloid Leukemia Pathway 83116

ncbi summary :

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]

size :

7 ml

price :

220 USD