product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Nitric Oxide Synthase-Endothelial (eNOS) Polyclonal Antibody
catalog :
MBS300695
quantity :
1 ml
price :
410 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS300695
products type :
Antibody
products full name :
Rabbit Anti-Human Nitric Oxide Synthase-Endothelial (eNOS) Polyclonal Antibody
products short name :
Nitric Oxide Synthase-Endothelial
other names :
nitric oxide synthase, endothelial isoform 1; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; endothelial NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 (endothelial cell); Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
products gene name :
eNOS
other gene names :
NOS3; NOS3; eNOS; ECNOS; cNOS; eNOS; NOSIII
uniprot entry name :
NOS3_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human
sequence length :
1203
form :
1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:70. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:70. IHC Positive Control: Placenta, Lung, Ovarian Carcinoma
other info2 :
Immunogen: Synthetic peptide derived from C-terminus of human e-NOS. Control Tissue: Placenta. Cellular Localization: Cytoplasm. Entrez Gene Code: 4846. Pathway: Synthesis and Degradation
products description :
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with eNOS.
ncbi gi num :
40254422
ncbi acc num :
NP_000594.2
ncbi gb acc num :
NM_000603.4
ncbi mol weight :
135 kDa
ncbi pathways :
ACE Inhibitor Pathway 198763!!AGE/RAGE Pathway 698754!!Angiogenesis Pathway 198772!!Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Corticotropin-releasing Hormone Pathway 920957!!Disease Pathway 530764
ncbi summary :
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
size :
1 ml
price :
410 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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