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antibody

Rabbit Anti-Human WILM'S Tumor (WT) Polyclonal Antibody

MBS300674

1 ml

410 USD

polyclonal

rabbit

rat, mouse, human

immunohistochemistry - paraffin section, immunohistochemistry

MBS300674

Antibody

Rabbit Anti-Human WILM'S Tumor (WT) Polyclonal Antibody

Wilm's Tumor

Wilms tumor protein isoform A; Wilms tumor protein; Wilms tumor protein; amino-terminal domain of EWS last three zinc fingers of the DNA-binding domain of WT1; Wilms tumor 1; WT33

WT

WT1; WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; N/A

WT1_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Dog, Mouse, Pig, Rabbit, Rat

497

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Wilm's Tumor

Immunogen: Synthetic peptide from C-terminus of human Wilm's tumor protein. Control Tissue: Wilm's Tumor. Cellular Localization: Nucleus. Entrez Gene Code: 7490. Pathway: Cell Junctions

Wilm's Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelioma.

65507714

NP_000369.3

NM_000378.4

47-55 kDa

Integrated Pancreatic Cancer Pathway 711360!!Regulation Of Telomerase Pathway 137987!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Oct 2010]

1 ml

410 USD