MyBioSource

antibody

Rabbit Anti-Human OCT-1 Polyclonal Antibody

MBS300644

1 ml

410 USD

polyclonal

rabbit

mouse, human, rat

immunohistochemistry, immunohistochemistry - paraffin section

MBS300644

Antibody

Rabbit Anti-Human OCT-1 Polyclonal Antibody

1-Oct

POU domain, class 2, transcription factor 1 isoform 2; POU domain, class 2, transcription factor 1; POU domain, class 2, transcription factor 1; NF-A1; OTF-1; Octamer-binding transcription factor-1; oct-1; octamer-binding protein 1; octamer-binding transcription factor 1; POU class 2 homeobox 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1

1-Oct

POU2F1; POU2F1; OCT1; OTF1; oct-1B; OCT1; OTF1; Oct-1; OTF-1

PO2F1_HUMAN

Polyclonal

IgG

Rabbit

Human. Predicted Reactivity: Chicken, Frog, Mouse, Rat

755

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Tonsil

Immunogen: Synthetic peptide derived from C-terminus of human OCT-1. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 5451. Pathway: Signal Transduction

The POU dimer from the Oct-1 gene formed on the palindromic Oct factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. The Oct-1 and 2 map to human chromosomes 1q22-q23 and 19, respectively.

311771667

NP_001185712.1

NM_001198783.1

80 kDa

ATF-2 Transcription Factor Network Pathway 138006!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!FOXA1 Transcription Factor Network Pathway 137979!!Gene Expression Pathway 105937!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway 160948!!RNA Polymerase III Abortive And Retractive Initiation Pathway 160949!!RNA Polymerase III Transcription Pathway 106561!!RNA Polymerase III Transcription Initiation Pathway 106562

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

1 ml

410 USD