Rabbit Anti-Human STAT3 Polyclonal Antibody | MyBioSource MBS300613 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human STAT3 Polyclonal Antibody

catalog :

MBS300613

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300613

products type :

Antibody

products full name :

Rabbit Anti-Human STAT3 Polyclonal Antibody

products short name :

STAT3

other names :

signal transducer and activator of transcription 3 isoform 2; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor; signal transducer and activator of transcription 3 (acute-phase response factor); Acute-phase response factor

products gene name :

STAT3

other gene names :

STAT3; STAT3; APRF; HIES; ADMIO; APRF

uniprot entry name :

STAT3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Frog, Mouse, Pig, Rat

sequence length :

769

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with thisprimary antibody.Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Thedilutions are estimates; actual results may differ because of variability in methods and protocols.Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed bycooling at room temperature for 20 min.Primary Antibody Incubation: Incubate for 10 minutes at room temperature.Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.Visualization: Detect the antibody as instructed by the instructions provided with the visualizationsystem. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Breast Carcinoma

other info2 :

Immunogen: Recombinant protein encoding aa 50-240 of human Stat3. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 6774. Pathway: Signal Transduction

products description :

The Stat3 protein is a transcription factor expressed in most cell types and is activated by multiplecytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, IL-12, IL-2, EGF etc. Stat3 binds toDNA as either a homodimer or as a heterodimer with Stat1. Stat3 interacts with the APRE (acutephase response element) and SIE (c-sis inducible element).

ncbi gi num :

21618338

ncbi acc num :

NP_003141.2

ncbi gb acc num :

NM_003150.3

ncbi mol weight :

89 kDa

ncbi pathways :

AGE/RAGE Pathway 698754!!Acute Myeloid Leukemia Pathway 83117!!Acute Myeloid Leukemia Pathway 529!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Androgen Receptor Signaling Pathway 198806!!B Cell Receptor Signaling Pathway 198909!!BDNF Signaling Pathway 712093!!CXCR4-mediated Signaling Events Pathway 137910

ncbi summary :

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

size :

7 ml

price :

220 USD