MyBioSource

antibody

Rabbit Anti-Human Alpha-1-Antichymotrypsin Polyclonal Antibody

MBS300566

1 ml

300 USD

polyclonal

rabbit

immunohistochemistry, immunohistochemistry - paraffin section

MBS300566

Antibody

Rabbit Anti-Human Alpha-1-Antichymotrypsin Polyclonal Antibody

alpha-1-Antichymotrypsin

alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin; alpha-1-antichymotrypsin; cell growth-inhibiting gene 24/25 protein; growth-inhibiting protein 24; growth-inhibiting protein 25; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; Cell growth-inhibiting gene 24/25 protein; Serpin A3Alpha-1-antichymotrypsin His-Pro-less

SERPINA3; SERPINA3; ACT; AACT; GIG24; GIG25; AACT; ACT

AACT_HUMAN

Polyclonal

IgG

Rabbit

Human

423

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: No special pretreatment necessary. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil

Immunogen: Purified human alpha-1-antichymotrypsin. Control Tissue: Tonsil. Cellular Localization: Cytoplasm. Entrez Gene Code: 12. Pathway: Enzymes

Alpha 1-ACT is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain. Regulation of the serine proteases and serine protease inhibitors plays an important role in neuromuscular differentiation. Prostate specific antigen (PSA), a chymotrypsin-like serine protease, is predominantly complexed to Alpha-1-ACT.

50659080

NP_001076.2

NM_001085.4

70 kDa

Selenium Pathway 198825

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]

1 ml

300 USD