Rabbit Anti-Human GAD65 Polyclonal Antibody | MyBioSource MBS300539 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human GAD65 Polyclonal Antibody

catalog :

MBS300539

quantity :

7 mL

price :

265 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300539

products type :

Antibody

products full name :

Rabbit Anti-Human GAD65 Polyclonal Antibody

products short name :

Glutamic Acid Decarboxylase 65

other names :

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

products gene name :

GAD65

other gene names :

GAD2; GAD2; GAD65; GAD65; GAD-65

uniprot entry name :

DCE2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Dog, Mouse, Pig, Rat

sequence length :

585

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Pancreas

other info2 :

Immunogen: Synthetic peptide from C-terminus of human GAD65. Control Tissue: Pancreas. Cellular Localization: Cytoplasm. Entrez Gene Code: 2572. Pathway: Stem Cell

products description :

Adults express two forms of glutamate decarboxylase (GAD), GAD65 and GAD67, which are encoded by different independently regulated genes. Both are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve endings; while GAD67 is more widely distributed throughout the cell. GAD65 preferentially synthesizes GABA for vesicular release and GAD67 preferentially synthesizes cytoplasmic GABA. GAD65 is predominantly expressed in pancreatic beta cells and auto-antibodies to GAD65 which are strongly associated with insulin-dependent diabetes mellitus (IMDM).

ncbi gi num :

4503875

ncbi acc num :

NP_000809.1

ncbi gb acc num :

NM_000818.2

ncbi mol weight :

65 kDa

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

ncbi summary :

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

uniprot summary :

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. Protein type: EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Lyase; Carbohydrate Metabolism - butanoate. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cytosol; cell junction. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding. Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

size1 :

7 mL

price1 :

265 USD